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nexteraxt dna sample preparation kit  (Illumina Inc)


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    Structured Review

    Illumina Inc nexteraxt dna sample preparation kit
    Nexteraxt Dna Sample Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xt+dna+sample+preparation+kit/TG+Nextera+XT+DNA+Sample+Preparation+Kit/pmc13049501-101-13-12
    Average 99 stars, based on 1645 article reviews
    nexteraxt dna sample preparation kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sample Prep:

    Article Title: Impaired neutralizing antibody responses against the BA1.1, BA.2, and BA.5 Omicron SARS-CoV-2 subvariants in hospitalized adults infected with pre-Omicron variants in Argentina
    Article Snippet: Briefly, total RNA extracted from nasopharyngeal swab specimens underwent complementary DNA synthesis with random hexamers using ProtoScript II (New England Biolabs, E6560), followed by whole-genome amplification with custom-designed tiling primers. .. Library preparation was performed using the Nextera XT DNA Sample Preparation Kit (Illumina, FC-131-1096). .. The Nextera XT libraries were sequenced in a paired-end 2 × 150 nt run format on the Illumina MiSeq platform.

    Article Title: Novel Reassortant H5N2 Highly Pathogenic Avian Influenza Viruses from Backyard Poultry in Mexico.
    Article Snippet: .. DNA libraries for next-generation sequencing were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced by generating multiplexed paired-end sequencing libraries [15]. ..

    Article Title: Mitochondrial genomes and phylogeny of Atratus and Educator Group species of the Melanoconion Section of Culex ( Melanoconion ) (Diptera: Culicidae)
    Article Snippet: Thermal cycler conditions were 94oC for 2 min, 42 cycles of 94oC for 30 s, 45oC for 20 s and 65oC for 13 min and a final extension at 72oC for 7 min. Amplicons were purified using DNA Clean & Concentrador TM (Zymo Research, California, USA) and quantified using a Qubit 2.0 fluorometer (LifeTechnologies, Oregon, USA), according to the manufacturer's instructions. .. Long PCR amplicon libraries were prepared using the Nextera® XT DNA Sample Preparation Kit (Illumina, Illinois, USA) and paired-end fragments (150 bp) were sequenced on the Illumina MiSeq platform. ..

    Article Title: Candesartan cilexetil disrupts methicillin-resistant Staphylococcus aureus membrane and potentiates gentamicin and polymyxin B activity.
    Article Snippet: Total genomic DNA was extracted from cultures of each strain using the DNeasy Blood & Tissue kit (Qiagen, Valencia, CA, USA) and quantified by Qubit dsDNA High-Sensitivity Assay (Invitrogen, Carlsbad, CA, USA). .. DNA AR TI CL E IN P RE SS libraries were prepared from 50 ng of DNA per sample using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) with recommended modifications for 2 x 250 bp paired-end sequencing. ..

    Article Title: Pangenome-based design of strain-specific primers enables precise monitoring of bacteria in human microbiome intervention trials
    Article Snippet: .. Whole-genome sequencing was performed using the Nextera XT DNA Sample Preparation Kit (Illumina) and the Illumina MiSeq platform (2 × 250 cycles) at the Laboratory of Medical Microbiology (University of Antwerp, Antwerp, Belgium), following a workflow described by Wuyts et al. . Genome assembly was performed using the Shovill pipeline 1.1.0 (github.com/tseemann/shovill), and quality control was assessed with CheckM and GUNC . ..

    Article Title: Novel Reassortant H5N2 Highly Pathogenic Avian Influenza Viruses from Backyard Poultry in Mexico
    Article Snippet: .. DNA libraries for next-generation sequencing were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced by generating multiplexed paired-end sequencing libraries [ ]. ..

    Article Title: Reciprocal regulation of fibroblast–macrophage equilibrium governs skin integrity
    Article Snippet: A cDNA library was generated from 2 ng of total RNA using the Smart-Seq V4 Ultra Low Input RNA Kit (Takara). .. A total of 150 pg of cDNA was used to make sequencing libraries with the Nextera XT DNA Sample Preparation Kit (Illumina). .. Libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).

    Article Title: A novel parvovirus circulating in canine populations and sporadically detected in human oropharyngeal samples
    Article Snippet: These nucleic acid samples containing DNA and RNA viral sequences were used for reverse transcription reactions with the SuperScript III reverse transcriptase (Invitrogen) and 100 pmol of a random hexamer primer, followed by a single round of DNA synthesis using Klenow fragment polymerase (New England BioLabs). .. Libraries were constructed using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina NovaSeq platform with 150 bp paired-end reads and dual barcoding. ..

    Next-Generation Sequencing:

    Article Title: Novel Reassortant H5N2 Highly Pathogenic Avian Influenza Viruses from Backyard Poultry in Mexico.
    Article Snippet: .. DNA libraries for next-generation sequencing were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced by generating multiplexed paired-end sequencing libraries [15]. ..

    Article Title: Novel Reassortant H5N2 Highly Pathogenic Avian Influenza Viruses from Backyard Poultry in Mexico
    Article Snippet: .. DNA libraries for next-generation sequencing were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced by generating multiplexed paired-end sequencing libraries [ ]. ..

    Sequencing:

    Article Title: Novel Reassortant H5N2 Highly Pathogenic Avian Influenza Viruses from Backyard Poultry in Mexico.
    Article Snippet: .. DNA libraries for next-generation sequencing were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced by generating multiplexed paired-end sequencing libraries [15]. ..

    Article Title: Candesartan cilexetil disrupts methicillin-resistant Staphylococcus aureus membrane and potentiates gentamicin and polymyxin B activity.
    Article Snippet: Total genomic DNA was extracted from cultures of each strain using the DNeasy Blood & Tissue kit (Qiagen, Valencia, CA, USA) and quantified by Qubit dsDNA High-Sensitivity Assay (Invitrogen, Carlsbad, CA, USA). .. DNA AR TI CL E IN P RE SS libraries were prepared from 50 ng of DNA per sample using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) with recommended modifications for 2 x 250 bp paired-end sequencing. ..

    Article Title: Pangenome-based design of strain-specific primers enables precise monitoring of bacteria in human microbiome intervention trials
    Article Snippet: .. Whole-genome sequencing was performed using the Nextera XT DNA Sample Preparation Kit (Illumina) and the Illumina MiSeq platform (2 × 250 cycles) at the Laboratory of Medical Microbiology (University of Antwerp, Antwerp, Belgium), following a workflow described by Wuyts et al. . Genome assembly was performed using the Shovill pipeline 1.1.0 (github.com/tseemann/shovill), and quality control was assessed with CheckM and GUNC . ..

    Article Title: Novel Reassortant H5N2 Highly Pathogenic Avian Influenza Viruses from Backyard Poultry in Mexico
    Article Snippet: .. DNA libraries for next-generation sequencing were prepared using the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) and sequenced by generating multiplexed paired-end sequencing libraries [ ]. ..

    Article Title: Reciprocal regulation of fibroblast–macrophage equilibrium governs skin integrity
    Article Snippet: A cDNA library was generated from 2 ng of total RNA using the Smart-Seq V4 Ultra Low Input RNA Kit (Takara). .. A total of 150 pg of cDNA was used to make sequencing libraries with the Nextera XT DNA Sample Preparation Kit (Illumina). .. Libraries were quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).

    Polymerase Chain Reaction:

    Article Title: Mitochondrial genomes and phylogeny of Atratus and Educator Group species of the Melanoconion Section of Culex ( Melanoconion ) (Diptera: Culicidae)
    Article Snippet: Thermal cycler conditions were 94oC for 2 min, 42 cycles of 94oC for 30 s, 45oC for 20 s and 65oC for 13 min and a final extension at 72oC for 7 min. Amplicons were purified using DNA Clean & Concentrador TM (Zymo Research, California, USA) and quantified using a Qubit 2.0 fluorometer (LifeTechnologies, Oregon, USA), according to the manufacturer's instructions. .. Long PCR amplicon libraries were prepared using the Nextera® XT DNA Sample Preparation Kit (Illumina, Illinois, USA) and paired-end fragments (150 bp) were sequenced on the Illumina MiSeq platform. ..

    Amplification:

    Article Title: Mitochondrial genomes and phylogeny of Atratus and Educator Group species of the Melanoconion Section of Culex ( Melanoconion ) (Diptera: Culicidae)
    Article Snippet: Thermal cycler conditions were 94oC for 2 min, 42 cycles of 94oC for 30 s, 45oC for 20 s and 65oC for 13 min and a final extension at 72oC for 7 min. Amplicons were purified using DNA Clean & Concentrador TM (Zymo Research, California, USA) and quantified using a Qubit 2.0 fluorometer (LifeTechnologies, Oregon, USA), according to the manufacturer's instructions. .. Long PCR amplicon libraries were prepared using the Nextera® XT DNA Sample Preparation Kit (Illumina, Illinois, USA) and paired-end fragments (150 bp) were sequenced on the Illumina MiSeq platform. ..

    Control:

    Article Title: Pangenome-based design of strain-specific primers enables precise monitoring of bacteria in human microbiome intervention trials
    Article Snippet: .. Whole-genome sequencing was performed using the Nextera XT DNA Sample Preparation Kit (Illumina) and the Illumina MiSeq platform (2 × 250 cycles) at the Laboratory of Medical Microbiology (University of Antwerp, Antwerp, Belgium), following a workflow described by Wuyts et al. . Genome assembly was performed using the Shovill pipeline 1.1.0 (github.com/tseemann/shovill), and quality control was assessed with CheckM and GUNC . ..

    Construct:

    Article Title: A novel parvovirus circulating in canine populations and sporadically detected in human oropharyngeal samples
    Article Snippet: These nucleic acid samples containing DNA and RNA viral sequences were used for reverse transcription reactions with the SuperScript III reverse transcriptase (Invitrogen) and 100 pmol of a random hexamer primer, followed by a single round of DNA synthesis using Klenow fragment polymerase (New England BioLabs). .. Libraries were constructed using the Nextera XT DNA Sample Preparation Kit (Illumina) and sequenced on the Illumina NovaSeq platform with 150 bp paired-end reads and dual barcoding. ..



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